Table 5 Results of stability among 15 candidate genes computed by 4 algorithms using 35 bottlenose dolphin blood samples.

From: Selection of suitable reference genes for normalization of quantitative RT-PCR in peripheral blood samples of bottlenose dolphins (Tursiops truncatus)

HKGs

Comprehensive Ranking

Delta CT

BestKeeper

NormFinder

geNorm

Geomean of Ranking Value

Rank

Average of SD

Rank

SD

Rank

Stability value

Rank

M value

Rank

HPRT1

1.86

1

0.603

2

0.522

1

0.334

1

0.371

6

PGK1

2.21

2

0.601

1

0.567

4

0.395

2

0.238

3

ACTB

4.20

3

0.644

4

0.675

13

0.489

6

0.225

1

RPL4

4.21

4

0.616

3

0.580

5

0.395

3

0.485

7

LDHA

4.74

5

0.659

7

0.644

9

0.513

8

0.225

1

RPS9

5.57

6

0.648

5

0.580

6

0.453

4

0.549

8

B2M

5.73

7

0.680

9

0.540

2

0.468

5

0.635

12

GAPDH

7.70

8

0.682

10

0.637

8

0.532

11

0.259

4

RPS18

7.84

9

0.659

6

0.645

10

0.508

7

0.593

9

TFRC

8.44

10

0.712

13

0.541

3

0.525

10

0.644

13

RPL8

10.19

11

0.675

8

0.688

15

0.523

9

0.614

10

YWHAZ

10.22

12

0.705

12

0.681

14

0.566

13

0.284

5

RPL18

11.24

13

0.685

11

0.645

11

0.552

12

0.624

11

SDHA

12.40

14

0.760

15

0.615

7

0.591

15

0.672

15

LDHB

13.47

15

0.754

14

0.662

12

0.576

14

0.659

14