Table 5 Results of stability among 15 candidate genes computed by 4 algorithms using 35 bottlenose dolphin blood samples.
HKGs | Comprehensive Ranking | Delta CT | BestKeeper | NormFinder | geNorm | |||||
---|---|---|---|---|---|---|---|---|---|---|
Geomean of Ranking Value | Rank | Average of SD | Rank | SD | Rank | Stability value | Rank | M value | Rank | |
HPRT1 | 1.86 | 1 | 0.603 | 2 | 0.522 | 1 | 0.334 | 1 | 0.371 | 6 |
PGK1 | 2.21 | 2 | 0.601 | 1 | 0.567 | 4 | 0.395 | 2 | 0.238 | 3 |
ACTB | 4.20 | 3 | 0.644 | 4 | 0.675 | 13 | 0.489 | 6 | 0.225 | 1 |
RPL4 | 4.21 | 4 | 0.616 | 3 | 0.580 | 5 | 0.395 | 3 | 0.485 | 7 |
LDHA | 4.74 | 5 | 0.659 | 7 | 0.644 | 9 | 0.513 | 8 | 0.225 | 1 |
RPS9 | 5.57 | 6 | 0.648 | 5 | 0.580 | 6 | 0.453 | 4 | 0.549 | 8 |
B2M | 5.73 | 7 | 0.680 | 9 | 0.540 | 2 | 0.468 | 5 | 0.635 | 12 |
GAPDH | 7.70 | 8 | 0.682 | 10 | 0.637 | 8 | 0.532 | 11 | 0.259 | 4 |
RPS18 | 7.84 | 9 | 0.659 | 6 | 0.645 | 10 | 0.508 | 7 | 0.593 | 9 |
TFRC | 8.44 | 10 | 0.712 | 13 | 0.541 | 3 | 0.525 | 10 | 0.644 | 13 |
RPL8 | 10.19 | 11 | 0.675 | 8 | 0.688 | 15 | 0.523 | 9 | 0.614 | 10 |
YWHAZ | 10.22 | 12 | 0.705 | 12 | 0.681 | 14 | 0.566 | 13 | 0.284 | 5 |
RPL18 | 11.24 | 13 | 0.685 | 11 | 0.645 | 11 | 0.552 | 12 | 0.624 | 11 |
SDHA | 12.40 | 14 | 0.760 | 15 | 0.615 | 7 | 0.591 | 15 | 0.672 | 15 |
LDHB | 13.47 | 15 | 0.754 | 14 | 0.662 | 12 | 0.576 | 14 | 0.659 | 14 |