Figure 5
Zwint-1 is required for Aurora B/C kinase to correct chromosome misalignment during meiosis.
Control or Zwint-1-knockdown oocytes were fixed at 8 h after GVBD or 6 h after GVBD, respectively and immunostained with either anti-Aurora C kinase (a) or anti-H3S10 ph (b) antibodies. Scale bar, 10 μm. Quantification of fluorescent intensity is shown in the right panel of images. (c) At 4 h after GVBD, oocytes were cultured with 10 μM ZM447439 for 5 h. To prevent premature PBE, 25 μM MG132 was added in the culture media. Oocytes were then washed out from ZM447439 and cultured for 2 h in the presence of MG132. Oocytes were fixed and immunostained with ACA and DAPI. Scale bar, 10 μm. (d) Quantification of chromosome misalignment was shown. Data are obtained from three independent experiments with the indicated number of oocytes (*p = 0.027; ns, not significant).
