Figure 7
Antagonists of nAchR and α7nAchR, but not of mAchR, abolish electroacupuncture-induced inhibition of myocardial HMGB1 expression after ischemia-reperfusion.
Atropine (Atro), mecamylamine (Mec) and methyllycaconitine (MLA) were administered to mice that were later subjected to electroacupuncture and ischemia-reperfusion. Animals were sacrificed and examined after 0 and 24 h of reperfusion. (A,B) Levels of HMGB1 protein in left ventricle tissue were determined by Western blot. Representative results and densitometric analysis are shown (n = 4). (C,D) Levels of HMGB1 mRNA in left ventricle tissue were determined by quantitative PCR. Representative results are shown with respect to PC6+IR (n = 4). Data are mean ± SEM. *p < 0.05 vs. IR; #p < 0.05 vs. PC6+IR (one-way ANOVA followed by Bonferroni correction). All data come from 3 independent experiments.
