Figure 8
Acetylcholine, but not noradrenaline, inhibits hypoxia-induced release of HMGB1 from primary cardiomyocytes, which depends on nAchR, primarily α7nAchR.
Neonatal rat cardiomyocytes were cultured under hypoxic conditions for 6 h in the presence of different concentrations of acetylcholine (Ach) or noradrenaline (NE). (A) Levels of HMGB1 in the culture medium were determined by ELISA (n = 4). (B) Experiments were repeated by co-administering 10 μM acetylcholine with atropine (Atro), mecamylamine (Mec) or methyllycaconitine (MLA) (n = 4). Data are mean ± SEM. *p < 0.05 vs. Normoxia; #p < 0.05 vs. Hypoxia; &p < 0.05 vs. H+Ach (one-way ANOVA followed by Bonferroni correction). All data come from at least 3 independent experiments.
