Figure 4
Photoacoustic “nanobombs” amplify therapeutic efficacy of DOX in vitro and in vivo.
(a) In vitro cytotoxicity of DOX with and without SWCNTs/laser. Means ± SD are shown (n = 6). The laser irradiation was 15 min at 100 mW cm−2 and the SWCNT concentration was 10 μg ml−1. (b) The growth curves of A549 tumors on mice receiving different therapeutic formulations. Means + SD are shown (n = 5 per group). The laser irradiation was 1 min at 600 mW cm−2 and the SWCNT concentration was 1.5 mg kg−1. (c) Representative images of A549 tumor-bearing mice (left panel) and the separated tumor tissues after treatment (right panel). (d) The weight of tumor tissues after different treatments. Means + SD are shown (n = 5 per group). (e) Body weight variation of the mice during the treatment. Means + SD are shown (n = 5 per group). (f) Tumor sections were processed with hematoxylin (for nuclear staining, dark purple dots) and eosin (for cytoplasm counterstaining, deep pink). Black arrows head depict necrotic areas containing less or no cells. Scale bars = 100 μm.
