Table 1 Summary of results from this work.
Protein | Rigid loop? | Fibril formation | Oligomer formation | Thermal denaturation | Urea-induced denaturation | Molecular dynamics |
|---|---|---|---|---|---|---|
recMoPrP | No | Rapid | Slow | Medium | Stable | Loop region can adopt β-turn or 3–10 helix; Tyr168 can be either solvent exposed or protected |
recMoPrP-S169N | Yes | Slow | Rapid | Less stable | Less stable | Reduced overall stability; Loop region can adopt β-turn or 3–10 helix; Tyr168 can be either solvent exposed or protected |
recMoPrP-N173T | No | Rapid | Slow | Medium | Stable | Loop region can adopt β-turn or 3–10 helix; Tyr168 only solvent protected |
recMoPrP-S169N/N173T | Yes | Slow | Very rapid and large oligomers formed | More stable | Less stable | Loop region only adopts a β-turn; Tyr168 only solvent exposed |
