Figure 1 | Scientific Reports

Figure 1

From: Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX

Figure 1

CE-SELEX selection and Electropherogram.

(A,B) Capillary electropherogram of ssDNA library or AFP protein in free-solution, respectively. (C) Capillary electropherogram of separated unbound ssDNA and AFP-bound ssDNA. (D) Identification of ssDNA and PCR products using 8% PAGE/7 M urea gel electrophoresis. Lane 1: 50 bp DNA Ladder; lane 2: ssDNA sub-library generated by streptavidin-coated magnetic beads; lane 3: initial library; lane 4: PCR products. The shift of ssDNA should refer to the position of initial library, which is different from dsDNA.

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