Figure 8
Effect of LDH-A inhibition on the expression of metabolic genes and on ATP/ADP ratio.
(a–c) U87MG or U87MG-GSCs were treated with DMSO (control), or NHI-1 or NHI-2 (1 μM−50 μM) for 48 h or 7 days, respectively. At the end of the treatment periods, the mRNA amount of HIF-1α, PKM2, GLUT1 and LDH-A was quantified using real time RT-PCR. The data were expressed as the fold change relative to the level of expression in GBM cells or control set to 1 and are the mean values ± SEM of three different experiments performed in duplicate. (d,e) U87MG cells or U87MG-GSCs were treated with DMSO (control), or NHI-1 or NHI-2 (1 μM−10 μM), or DCA (100 μM) for 48 h or 7 days. At the end of treatments, ATP and ADP levels were quantified by a luminescence assay kit. The data were expressed as ATP levels (d) or ATP/ADP ratio (e) and are the mean values ± SEM of three different experiments performed in triplicate. The significance of the differences is determined with a one-way ANOVA with Bonferroni’s post hoc test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. respective control; ##P ≤ 0.01 vs. the relative expression in U87MG cells.
