Figure 3
From: Mesenchymal Stem Cells Reduce Murine Atherosclerosis Development
MSC treatment affects CD8+ T cell and monocyte responses in vivo.
(a) CD8+ T cells were measured in the circulation throughout the entire experiment by flow cytometry. (b) After eight weeks, effector CD8+ T cells, determined as CD62L− within CD8+ T cells, in the circulation and spleen were determined by flow cytometry. (c) Splenocytes were isolated and stimulated with αCD3/CD28 for 72 hours. Proliferation was assessed by the amount of 3H-thymidine incorporation. Proliferation is normalized for proliferation of controls (without stimulation) and expressed as the stimulation index (S.I.). (d) Circulating monocytes, determined as CD11bhiLy-6G−, were analyzed by flow cytometry. CCL2 levels in serum were determined by ELISA. All values are expressed as mean ± SEM and representative of six mice per group. *P < 0.05, **P < 0.01.
