Figure 2
From: Subdiffraction localization of a nanostructured photosensitizer in bacterial cells
Improvement in resolution by STED microscopy.
(A,B) Comparison between B. subtilis images collected with confocal microscopy (A) and with STED nanoscopy (STED power 30 mW, pixel dwell time 0.1 ms) (B). (C) The blue and the red intensity profiles were measured along the segment connecting the arrows in (A,B) respectively. Scale bars are 2.5 μm. (D) Fluorescence depletion curves for Hyp in DMSO (10 μM, orange circles; Is = 3.1 ± 0.1 mW, α = 0.10 ± 0.01 (see Supporting Information for definition of parameters) and Hyp-apoMb in PBS (10 μM Hyp, 30 μM apoMb, blue circles; Is = 6.5 ± 0.1 mW, α = 0.14 ± 0.01) collected under excitation at 566 nm and detection at 605/70 nm. The STED beam was at 715 nm. Solid lines are the best fit to depletion functions (Equation 2).
