Figure 2
Transcriptional analysis of genes encoding CAZymes and related CBMs during different developmental stages of S. sclerotiorum.
(a) The expression levels of genes encoding PCW- and FCW-degradation-associated CAZymes and respective related CBMs during different developmental stages. The expression levels were measured using the geometric mean of the TPM values of all genes in corresponding groups. To calculate the geometric mean of TPM values, all the TPM values of 0 were replaced by 0.001. (b) Gene expression cluster analysis of genes encoding PCW-degradation-associated CAZymes and related CBMs. (c) Gene expression cluster analysis of genes encoding FCW-degradation-associated CAZymes and related CBMs. The TPM values were used for the gene expression cluster analysis. Red, green and grey indicate high expression, low expression and no expression, respectively; For each heat map: top, stage tree; left, gene tree. Expression values are indicated in log2 scale. Column diagrams show the proportions of differentially expressed genes and unchanged expressed genes encoding PCW- (d) and FCW- (e) degradation-associated CAZymes and respective related CBMs, during different developmental stages, compared with the vegetative growth stage of S. sclerotiorum. The differentially expressed genes in the DGE data were identified with the threshold of |log2Ratio| ≥ 1 and FDR ≤ 0.001. Up, Down and Un indicate the up-regulated, down-regulated and unchanged expressed genes, respectively.
