Figure 2 | Scientific Reports

Figure 2

From: Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx

Figure 2

DH and glycogen contents in ΔDHP and ΔDHR mutants.

(A–J) Representative images of immunohistochemical staining of pupal subesophageal ganglion (SG) using anti-DH[N] (A,C,E,G,I) and anti-PBAN[N] (B,D,F,H,J). The pupal SG was dissected 4 d after pupation and immunostaining was performed in the wt (25DD) (A,B), ΔDHP33 (25DD) (C,D), ΔDHP531 (25DD) (E,F), ΔDHP96 (25DD) (G,H) and ΔDHR111 (25DD) (I,J). The DHPC somata (SMd, SMx, SLb and SL) were detected in wt and ΔDHR. (K) The DH titer in the hemolymph was measured 4 d after pupation, by using TR-FIA. Each bar represents the mean ± SD of 8 samples. N.D., not detected; **P < 0.01; ***P < 0.001 vs. wt (25DD). (L) Glycogen content was measured in ovaries just after eclosion. Each bar represents the mean ± SD of 8 pupae. n.s., not significant vs. wt (15DD); ***P < 0.001 vs. wt (25DD). (Scale bar, 100 μm).

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