Figure 3
From: Zebrafish Models for Human Acute Organophosphorus Poisoning
Motor behaviour is strongly impaired by chlorpyrifos-oxon in zebrafish larvae.
(A) VMR profiles, showing that the hyperactivity peak evoked in response to sudden exposure to darkness is reduced until total abolition when 8 days post fertilization zebrafish larvae were previously exposed to increasing concentrations of CPO for 24 h. (B) Locomotion tracking plots for the VMR assay in a 48-well behavioural arena (1 larva per well) recorded a decrease in the swimming activity with increasing chlorpyrifos-oxon concentrations during the first 2 min of the light/dark challenge (30–32 min of the assay). (C,D) Kinematics of TMR traces for control (C) and grade 1 (D) larvae. For each condition, six representative traces are shown from the first 150 ms of the escape response. Each trace is from a different larva. The curvature of the body is represented in degrees, with 0 indicating a straight body. Only grade 1 larvae without any morphological defects were selected for behavioural analyses.
