Figure 3
From: Runx2 contributes to the regenerative potential of the mammary epithelium
RUNX2 expression is enriched during mammosphere culture.
(A) qRT-PCR for Runx2 on mouse mammary epithelial cells grown in 2D (2D MMECs), primary and secondary mammospheres. Gene expression is shown as relative expression to Gapdh (mean ± SD). n ≥ 5 for each group. Expression in primary and secondary mammospheres was compared to 2D MMECs; *p < 0.05. **p < 0.0001 (ANOVA with post-hoc Bonferroni correction). Expression in primary mammospheres was also compared to secondary mammospheres; *p < 0.05 (ANOVA with post-hoc Bonferroni correction). (B) Western blot of MMECs grown in 2D and primary mammospheres from three independent experiments. GAPDH used as loading control. (C) Representative RUNX2 immunocytochemistry of primary and secondary mammospheres. Scale bars represent 30 μm.
