Figure 4
From: Runx2 contributes to the regenerative potential of the mammary epithelium
RUNX2 potentiates mammosphere-forming potential of HC11 cells.
(A) qRT-PCR for Runx2 on HC11 grown in 2D and as mammospheres (mammo). Gene expression is shown as relative expression to Gapdh (mean ± SD); n ≥ 3 for each group. Expression in mammospheres was compared to 2D MMECs; *p = 0.02 (Unpaired t-test with Welch’s correction). (B) Western blot of HC11 cells transfected with empty vector (HC11-CTR) and RUNX2 overexpressing plasmid (HC11-Runx2). GAPDH used as loading control. (C) Growth curve of HC11-CTR and HC11-Runx2 cells grown in 2D culture. Cell numbers were counted daily in triplicate for each time point, for each cell line. Data are expressed as mean +/− SD. Graph is representative of two independent experiments from 2 independent lines of HC11-CTR and 3 independent lines of HC11-Runx2. (D) Mammospheres from HC11-CTR and HC11-Runx2 cells grown in non-adherent conditions for 7 days. Data are expressed as mean +/− SD. Graph representative of four independent experiments from 2 independent lines of HC11-CTR and 3 independent lines of HC11-Runx2. The number of mammospheres formed by HC11-CTR and HC11-Runx2 was compared. *p < 0.0001 (Unpaired t-test with Welch’s correction).
