Figure 6
From: Runx2 contributes to the regenerative potential of the mammary epithelium
RUNX2 contributes to activation of WNT signalling transcriptional program in mammary cells.
qRT-PCR for Axin2 (A) and Runx2 (B) on primary mammospheres (1st Mammo) treated for 24h, with either vehicle (Vh) or recombinant WNT3a. 3 independent MMEC extractions for each group. *p < 0.05. **p < 0.0005 (Unpaired t-test with Welch’s correction). Gene expression is shown as relative expression to Gapdh (mean ± SD). (C) qRT-PCR for Runx2 on RNA extracted from BLG-Cre:Catnb+/+ and BLG-Cre:Catnb+/lox(ex3) virgin glands. n ≥ 3 for each group. *p < 0.05 (Unpaired t-test with Welch’s correction). (D) RUNX2 immunohistochemistry on lactating day 1 glands from BLG-Cre:Catnb+/+ and BLG-Cre:Catnb+/lox(ex3) mice, scale bar represents 50 μM. (E) qRT-PCR for Runx2 on RNA extracted from HC11 cells treated for 24 h, with either vehicle (Vh) or WNT3a. n = 5 for each group. *p = 0.01 (Paired t-test). Gene expression is shown as relative expression to Gapdh. Data are expressed as mean fold expression (±SD). (F,G) qRT-PCR for Axin2, CyclinD1 and Sox9 on RNA extracted from HC11 cells transfected with either scrambled (siSCR) or Runx2 targeted siRNA (siRunx2) and treated for 24 h with WNT3a. n = 5 independent experiments for each group. *p < 0.05 **p < 0.005. (Paired t-test). Gene expression is shown as fold change relative to siSCR. Data for siRunx2 are expressed as mean fold expression (±SD).
