Figure 7
From: Runx2 contributes to the regenerative potential of the mammary epithelium
Exogenous WNT activation does not rescue Runx2-depleted mammospheres.
Bright field images of mammosphere cultures (A) derived from K14-Cre:Runx2wt/wt (top) and K14-Cre:Runx2fl/fl (bottom) mice, treated for 6 days with either vehicle (Vh) or recombinant WNT3a (100 ng/ml). Scale bars represent 100 μm. The average area of primary mammospheres (B) and number (area cut-off 4000 pixel) of primary spheres (C) of MMECs extracted from K14-Cre:Runx2wt/wt and K14-Cre:Runx2fl/fl mice, treated for 6 days with either vehicle (Vh) or WNT3a. Mammospheres were counted and measured after 6 days in culture. Data are expressed as mean (±SD). The area and number of mammospheres formed by K14-Cre:Runxwt/wt and K14-Cre:Runx2fl/fl MMECs after WNT3a treatment was compared. *p < 0.05; **p < 0.001 (Unpaired t-test with Welch’s correction).
