Figure 6

Safranin-O/Fast Green stained sections of annulus fibrosus tissue from a non-degenerative (a,c) and a degenerative (b,d) disc. Intact fibers with parallel arrangement are noted in the AF of a lumbar disc (degeneration Grade I) from a 13 year old male (c); while fibers are loose and arrangement is disrupted in the AF of a lumbar disc from a 47 year old female with disc degeneration Grade III (d). Scale bars: 500 μm (a,b); 100 μm (c,d).