Figure 2

GFP expression predominantly co-localizes with insulin-producing β-cells in adult islets.

(a) Immunohistochemistry analysis of paraffin sections from adult pPax4-Cre-IRES-Egfp mice pancreas. Representative microscopy images for co-immunofluorescent labeling of GFP (green) together with E-CADHERIN (for cellular delimitation) or with the pancreatic islet cell markers INSULIN (red), GLUCAGON (red) or SOMATOSTATIN (red). Nuclei counterstaining was performed using DAPI (blue). (b) Quantitative analysis (average ± SE) of the GFP⁺ cell population composition. (c) Quantification of the percentage of INSULIN⁺, GLUCAGON⁺ and SOMATOSTATIN⁺ cells that co-express GFP (average ± SE). Sections (2 to 4) from 3 to 5 independent animals with an average of 15 islets and 1400 cells per animal were used for quantifications.