Figure 8
From: Discovery of Small Molecules that Inhibit the Disordered Protein, p27Kip1
Interaction of p21-KID with representative p27 fragment hits.
(a) Comparison of amino acid sequences of the D2 subdomains of p27-KID and p21-KID; (b,c) Individual 1H (ΔδHN) chemical shift perturbations (through analysis of 2D 1H-15N HSQC NMR spectra) caused by addition of SJ319843 (b, Group 1) and SJ403 (c, Group 2), respectively, to 2H/15N-p21-KID (20 μM). The threshold for identifying specific binding interactions with the p27-KID residues was defined as two standard deviations above the average of chemical shift perturbations (Δδave+2σ, represented as the dotted black line) and considered if perturbations were larger than the experimental 1H digital resolution (shown as dotted magenta line).
