Figure 4
VAEM-STORM imaging of cortical microtubules in different parts of an intact Arabidopsis root.
(A) Mosaic of bright field images of the root tip. Seven regions (a-g) across the root tip are highlighted and were chosen for dSTORM imaging. (B) Zoomed-in bright field and corresponding epi-fluorescence and STORM images of the white-boxed regions in (A). Images are shown of cortical microtubules of cap cells (a), epidermal cells in meristematic zone (b), elongation zone (c-d), differentiation zone (g) and transition zones (e-f) between elongation and differentiation of the intact Arabidopsis root tip. Note that the zoomed-in bright field image, epi-fluorescence image and STORM image of cells in region dare shown in Fig. 1. Cell boundaries are highlighted with red dashed lines. Cells in regions fand g show distinct microtubule organization patterns, thus they are analyzed separately in Fig. 5 as f1, f2 and g1, g2 as indicated in the corresponding bright field images. Scale bars: 50 μm (A) and 3 μm (B, a-g).
