Figure 3

Dynamics of rapid protein dephosphorylation in mouse brains after death.
Mice were sacrificed by cervical dislocation and forebrains were dissected out. The times spent between the cervical dislocation and the brains put into dry ice were recorded. The phosphorylation levels of proteins determined by Western blots were plotted against brain collecting times. The non-linear regression was performed and the coefficient of determination (r2) was calculated with Graphpad prism 5.