Figure 3
From: Tiagabine Protects Dopaminergic Neurons against Neurotoxins by Inhibiting Microglial Activation
Neither muscimol nor baclofen affects MPTP-induced nigrostriatal dopaminergic toxicity.
Samples were collected from the striatum (A,B,D,E) and the substantia nigra (C,F) 1 day after drug treatment. (A,D) Western blot showing striatal TH protein levels. β-actin served as the loading control. Quantification of relative TH protein expression levels is shown in the lower panel. (B,E) Immunohistochemical staining showing striatal TH-ir nerve fibers. Densitometric analysis of the relative optical density of the staining is shown in the lower panel (scale bar: 0.2 mm). (C,F) Immunohistochemical staining showing TH-ir cells in the substantia nigra. The stereological counting of TH-ir cells is shown in the lower left panel and counting of the Nissl-positive neurons is shown in the lower right panel (scale bar: 0.2 mm). All data are presented as the mean ± SEM. *p < 0.05 and **p < 0.01 (n = 5–8).
