Figure 1 | Scientific Reports

Figure 1

From: Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E−/− Mice

Figure 1

The proteasome is activated in the abdominal aorta of human AAA tissue or Ang II-infused apo E−/− mice.

(a) The proteasome activities including caspase-like, trypsin-like and chymotrypsin-like activities were measured in aortic tissue from human. Activities are indicated as percentages of those in control aorta. (b) The expression of β2 and β2i was examined by immunohistochemistry. (c) The expression of β5i (red) and α-smooth muscle-actin (α-SMA) (green) was detected by double immunostaining. Nuclei were counterstained with DAPI (blue). (d) The proteasome activities were measured during the development of AAA in Apo E−/− mice. (e,f) The qPCR analysis for the mRNA expression of β1, β2, β5, β1i, β2i and β5i during the development of AAA in Apo E−/− mice. GAPDH was used as an internal control. Data expressed as means ± S.E.M. (n = 6 per group). *P < 0.05 vs Saline; ** P < 0.01 vs Saline. Scale bars: 50 μm. M: media; A: adventitia.

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