Figure 5 | Scientific Reports

Figure 5

From: Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E−/− Mice

Figure 5

Proteasome inhibitor BTZ inhibits the accumulation of immune cells and the expression of inflammatory cytokines in Ang II-infused apoE−/− mice.

(a) The representation of flow-cytometry analysis for quantifying the number of CD45+ immune cells in in abdominal aorta (left). Bar graph shows the percentage of CD45+ immune cells (right; n = 3 per group). (b) and (c) The representation of immunohistochemical staining for Mac-2 and CD4+ T cells in abdominal aorta from Apo E−/− mice (left). Bar graph shows the quantification of the percentage of Mac-2 and CD4+ T cell areas (right; n = 3 per group). (d) qPCR analysis for the mRNA expression of TNF-α, IFN-γ, IL-4, IL-6 and MCP-1 in abdominal aorta. GAPDH was used as an internal control. Magnification:×100. Scale bar: 50 μm. Data expressed as mean ± S.E.M. *P < 0.05, **P < 0.01 vs saline. #P < 0.05, ##P < 0.01 vs Ang II.

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