Figure 6

Proteasome inhibitor BTZ attenuates the proliferation of the aortic smooth muscle cells in Ang II-infused apoE−/− mice.
(a) pCNA (green) and α-SMA (red) double staining to detect proliferative SMCs in abdominal aorta from Apo E−/− mice (left). Nuclei were counterstained with DAPI (blue). Bar graph shows the percentage of pCNA positive areas (right, n = 3 per group). (b) TUNEL (green) and α-SMA (red) double staining to detect apoptotic SMCs (left). Bar graph shows the percentage of TUNEL positive areas (right, n = 3 per group). Data expressed as mean ± S.E.M. **P < 0.01 vs saline. ##P < 0.01 vs Ang II.