Figure 2
Vascular permeability analyses in non-tumor bearing tissue.
(A) VPIs for control and experimental reagents in non-tumor bearing CAMs represent the average value from 5 or 6 experiments run in triplicate (n = 15–18) at each concentration. Concentrations for control reagents are (1) 0.001 nM, (2) 0.01 nM, (3) 0.1 nM and (4) 1 nM; Concentrations for experimental reagents are (1) 0.005 nM, (2) 0.05 nM, (3) 0.1 nM and (4) 0.15 nM, (B) Comparison of average VPIs obtained with each control and VEA candidate under similar conditions in non-tumor bearing CAMs and rats using optimal concentrations determined in A. Average VPI values for the avian CAMs was determined from (PVL1, n = 56; H1-N, n = 41; PEP, n = 44; IL-2, n = 56; PVLs, 15–18). Average VPI values for the rat ears was determined from (PVL1, n = 5; H1-N, n = 3; PEP, n = 5; IL-2, n = 4; PVL2, n = 3; PVL5, n = 3; PVL10 n = 7). (C) Representative images for the controls and PVLs tested in the rat Miles assay. Ears were photographed prior to euthanasia and tissue processing.
