Table 1 Characteristics of VEA candidates tested with CAMs.
Reagent | Description | Binding Potency | Mol. Wt.*(Daltons) | pI* |
|---|---|---|---|---|
PVL 1 | Control: NHS76 antibody with an IgG2 constant region. No IL-2 fused to antibody. | 100% | 142,795.7 | 8.01 |
PVL 2 | Fusion of wildtype, mature IL-2 (133 amino acids) to the carboxy tail of NHS76 (IgG2). | 78.5% | 173,595.7 | 8.48 |
PVL 5 | Fusion of IL-2 residues 1–72 (includes full B-helix) with C-tail of NHS76 (IgG2). Point mutation of lysines 8 and 9 into alanines. Point mutation of cysteine 58 into valine. | 160.2% | 159,491.4 | 8 |
PVL 6 | Fusion of IL-2 residues 1–57 (includes partial B-helix corresponding to PEP fragment) to C-tail of NHS76 (IgG2). Point mutation of lysines 8 and 9 into alanines. | 78.4% | 155,851.2 | 8.48 |
PVL 9 | Fusion of IL-2 residues 1–51 (does not include B-helix) to C-tail of NHS76 (IgG2). Mutation of lysines 8 and 9 into alanines. | Lot 1:166.6% Lot 2:141.1% | 154,353.4 | 8.48 |
PVL 10 | Fusion of IL-2 residues 22–57 (PEP fragment) to C-tail of NHS76 (IgG2). A GGGGSGGGG linker was included between the NHS76 and the PEP fragment. | 90.6% | 151,610.4 | 8.63 |
