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Figure 1

From: Molecular architecture of the Ub-PCNA/Pol η complex bound to DNA

Figure 1

Map validation, overall structure of the 3D reconstruction and atomic model docking.

(a) Comparison between representative map projections (i) and the corresponding reference-free class averages (ii) in the same orientation to demonstrate self-consistency of the final map. (b) Tilt-pair test to validate the overall accuracy of the map and its absolute hand. Pairs of images were acquired at 0° and +10° for the Ub-PCNA/Pol η/DNA ternary complex and as control for Haliotis diversicolor hemocyanin isoform 1 (HdH1). The input model for the control tilt-pair test was created by low-pass filtering the previously published 4.5 Å resolution map (EMDB ID 5585) to 20 Å resolution. (c) Surface views of the final map. (d) The crystal structure of human PCNA (PDB ID 1VYM) was fitted to the bottom layer of the map. The scale bar represents 25 Å. (e) The crystal structure of the N-terminal catalytic core of Pol η (PDB ID 3MR2) was fitted to the top layer of the map. Unoccupied density corresponding to the C-terminal domain of Pol η is indicated with dark green arrows.

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