Figure 2
Compensated myocardial NAD+ concentration and Sirt1 activity in spite of decreased Nampt expression in TAC-operated hearts.
(a) The mRNA levels of Nampt in hearts at 8 w after TAC or sham operation (n = 5). (b) Immunoblot analysis of Nampt in hearts at 8 w after operation. The quantitations of the Nampt/GAPDH are shown as bar graphs (n = 5). (c) The mRNA levels of Sirt1 in hearts at 8 w after operation (n = 5). (d) Immunoblot analysis of Sirt1 in hearts at 8 w after operation. The quantitations of the Sirt1/GAPDH are shown as bar graphs (n = 5). (e) Sirt1 deacetylase activity in hearts at 8 w after operation (TAC, n = 5; sham; n = 8). (f) Immunoblot analysis of acetylated FoxO1 (Ac-FoxO1) and FoxO1 in hearts at 8 w after operation. The quantitations of the Ac-FoxO1/FoxO1 are shown as bar graphs (n = 4). (g) NAD+ concentrations (TAC, n = 10; sham, n = 11) and (H) NMN concentrations (TAC, n = 5; sham, n = 4) in hearts at 8 w after operation. *P < 0.05, **P < 0.01, NS, not significant.
