Figure 2
AnxA2 limits Kp spread in peritoneal cavity and circulating blood.
Groups of mice (n = 5/group) were anesthetized with ketamine (40 mg/Kg) and imaged at 0, 3, 6, 12 and 24 h after being dosed with 2 × 105 CFU bioluminescent Kp intraperitoneally. (a,b) Whole animal imaging of bioluminescence was detected by IVIS XRII system at different time points. It illustrated intense luminescence in red, moderate luminescence in green, low-level luminescence in blue, which indicated the severity of infection from strongest to weakest (n = 5; ANOVA; *P < 0.05). (c,d) 24 h after infection, imaging of extensively washed livers showed enhanced bacterial spread into livers of anxa2−/− mice (n = 5; ANOVA; *P < 0.05). (e,f) 24 h after infection, blood plating was cultured overnight and demonstrated a higher bacterial dissemination in the blood of anxa2−/− mice (n = 5; ANOVA; *P < 0.05).
