Figure 2
From: Maternal TET3 is dispensable for embryonic development but is required for neonatal growth
Effect of maternal TET3 depletion on 5mC and 5hmC levels in FG oocytes at the GV stage.
(a) Representative images showing the accumulation of DNMT3a in both SN and NSN types of nuclei in FG oocytes of [Zp3-cre, Tet3F/+] female mice. Oocytes were stained with anti-DNMT3a (red in merged images) and nuclei were revealed by staining of DNA with Sytox (green in merged images). Dashed circles indicate the rim of the oocyte. (b) Representative images showing the absence of maternal TET3 in both SN and NSN types of nuclei in FG oocytes of [Zp3-cre, Tet3F/+] or [Zp3-cre, Tet3F/–] female mice. Oocytes were stained with anti-mTET3(N) (red in merged images) and nuclei were revealed by staining of DNA with Sytox (green in merged images). Dashed circles indicate the rim of the oocyte. (c) Representative images showing the presence of 5hmC in both types of nuclei in FG oocytes of both [Zp3-cre, Tet3F/–] and [Zp3-cre, Tet3F/+] female mice. Nuclei were stained with anti-5hmC (red in merged images) and anti-5mC (green in merged images). Dashed circles indicate the rim of the nucleus.
