Figure 3
From: Maternal TET3 is dispensable for embryonic development but is required for neonatal growth
TET3 is dispensable for oocyte development, maturation and fertilization as well as for pregnancy.
(a) Number of MII stage oocytes obtained per ovulation for [Zp3-cre, Tet3F/+] (n = 10) and [Zp3-cre, Tet3F/–] (n = 10) female mice that were treated with PMSG and hCG. The number of ovulated MII stage oocytes for each mouse (circles) as well as the overall mean value (horizontal bar) and 95% confidence interval (CI) are shown in the left panel. The average difference in the number of ovulated MII stage oocytes between [Zp3-cre, Tet3F/+] and [Zp3-cre, Tet3F/–] female mice and its 95% CI are indicated in the right panel. Normality and homoscedasticity of the data were verified by the Shapiro-Wilk test (α = 0.05) and F test (α = 0.05), respectively. The P value was determined by the two-sided Student’s t test. (b) Oocytes obtained from [Zp3-cre, Tet3F/+] (n = 82) and [Zp3-cre, Tet3F/–] (n = 85) mice as in (a) were exposed to WT (ICR) spermatozoa in vitro for determination of the rate of fertilization, which was judged by the presence of two pronuclei in zygotes. The P value was determined by the one-sided Fisher’s exact probability test. (c) Strategy for evaluation of productive mating performed over a 2-month observation period. (d) [Zp3-cre, Tet3F/+] (n = 10) and [Zp3-cre, Tet3F/–] (n = 10) female mice were housed with WT (C57BL/6J) male mice as in (c) for determination of the number of matings (judged on the basis of plug formation) and the percentage of productive matings (based on the total number of litters produced relative to the total number of matings). The P value was determined by the one-sided Fisher’s exact probability test.
