Figure 4
From: Maternal TET3 is dispensable for embryonic development but is required for neonatal growth
TET3-mediated 5mC oxidation in the zygote is not required for mouse development.
(a,b) [Zp3-cre, Tet3F/+] (n = 10) or [Zp3-cre, Tet3F/–] (n = 10) female mice were housed with WT (C57BL/6J) males as in Fig. 3c and both litter size (a) and the number of weaned pups (b) were determined. Litter size and the number of weaned pups for each female (circles) as well as the overall mean values (horizontal bars) and 95% CIs are shown in the left panels and the average difference in litter size or the number of weaned pups between [Zp3-cre, Tet3F/+] and [Zp3-cre, Tet3F/–] dams and its 95% CI are indicated in the right panels. Normality and homoscedasticity of the data were verified by the Shapiro-Wilk test (α = 0.05) and F test (α = 0.05), respectively. The P values were determined by the two-sided Student’s t test. (c) The offspring produced in (a) ([Zp3-cre, Tet3F/+] dams, n = 74; [Zp3-cre, Tet3F/–] dams, n = 57) were counted at 7 days after birth and the percentages of live and dead pups were determined. The P value was determined by the one-sided Fisher’s exact probability test. (d) Tet3 genotype of weaned mice in (b) ([Zp3-cre, Tet3F/+] dams, n = 70; [Zp3-cre, Tet3F/–] dams, n = 46). He, heterozygous.
