Figure 2

Relationship between LVEDP and mTOR activity.

(a) Western blot of cell lysates obtained from heart tissue during 4 weeks of VO, probed for total and phosphorylated S6 and 4E-BP. (b) Relationship between diastolic wall stress and mTOR activity during 4 weeks of VO. (c) Left ventricular end diastolic pressure (LVEDP) at 24 h after induction of VO by using various needle sizes to penetrate the vein/artery (n = 3). (d) Western blot of cell lysates obtained from heart tissue at 24 h after VO induction using various needle sizes to penetrate the artery, probed for total and phosphorylated S6 and 4E-BP. (e) Relationship between LVEDP and S6 phosphorylation rate at 24 h after VO induction. GAPDH was used as a loading control. Data are shown as the mean ± SEM. **P < 0.01 vs. sham (Dunnett’s test).