Figure 6

(A) Chemical cross-linking of L-Asparaginase (30 μM) (MW: 36kDa, PDB ID: 4Q0M) at two different concentrations of glutaraldehyde. Resultant products were analyzed by 8% SDS-PAGE followed by Coomassie staining. (B) Chemical cross-linking of 2B peptide (15 μM) at two different concentrations of glutaraldehyde. Resultant products were analyzed by 16% Tris-Tricine SDS-PAGE followed by silver staining. Arrows indicate positions of monomeric and dimeric species. (C) Snapshots, at 0 (left panel), 5 (middle panel) and 100 ns (right panel), during MD simulation of four 2B peptides in water. The peptides interacting with each other are coloured red and blue respectively.