Figure 2

Construction and evaluation of SF14 and lacO fusion promoters.

(a) Sequences of SF14 and lacO fusion promoters. The putative transcription start sites are indicated by a bent arrow above the corresponding nucleotide. Sequences of lacO are underlined and coloured in light organge. The −10 and −35 regions are marked by dotted boxes. (b) Evaluation of SF14 and lacO fusion promoters on plates. After incubating the corresponding strains at 37 ^oC for 12 h, the plates were sprayed with catechol. (c) Dynamic expression profiles of SF14 and lacO fusion promoters measured by XylE activity induced with different IPTG concentrations. The values are presented as mean ± SD of three independent experiments.