Figure 4
From: A genetic biosensor for identification of transcriptional repressors of target promoters
Characterisation of the genetic biosensor.
(a) Evaluation of the genetic biosensor by an artificial library. The ScbR or ScbR2 rate indicates the positive rate of ScbR or ScbR2 clones identified among the total positive clones, respectively. The total rate is the sum of the ScbR and ScbR2 positive rates. The total numbers indicates the number of visible clones observed on plates. (b) Output of kanamycin resistance in response to target promoters with different binding ability. KD indicates the equilibrium dissociation constant of ScbR2 with the original or mutant kasO promoters. The plate contains 90 μg/ml kanamycin. (c) Output of XylE activity in response to target promoters with different binding ability. The values are presented as mean ± SD from three independent experiments. XylE activity of the strain with plasmids pCSW3-PkasO and pACW1 is set as control. Bridging lines show statistical details of comparisons between the control and the others. Aasterisks indicate the statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001)
