Figure 5

Identification of the selected repressors of PscbA.

(a) Schematic representation of the Lux reporter system. (b) CprA and CprB repress the bioluminescence controlled by PscbA in vivo. All values are in relative light units (RLU) and represent as mean ± SD from at least three independent experiments. Bioluminescence of the strain with plasmid pOscbAlux is set as control. Bridging lines show statistical details of comparisons between the control and the others. Asterisks indicate the statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001). (c) EMSA of the interaction of PscbA with CprA. Each lane contains 6.6 ng of PscbA probes and different amounts of CprA. Lanes 1–6 contain 0 nM, 3 nM, 10 nM, 30 nM, 0.1 μM, 0.3 μM of CprA, respectively. (d) EMSA of the interaction of PscbA with CprB. Each lane contains 6.6 ng of PscbA probes and different amounts of CprB. Lanes 1–7 contain 0 nM, 3 nM, 10 nM, 30 nM, 0.1 μM, 0.3 μM and 1 μM of CprB, respectively.