Figure 6

Selection of the repressors of Paco from a genomic DNA library.

(a) Genomic DNA fragments identified by sequencing. (b) XylE activity of the biosensor in response to repressors in the three cloned genomic DNA fragments. XylE activity of the strain with plasmid pACW2 is set as control. (c) EMSA of the interactions of Paco with Sav1778, Sav2270, Sav2268, AvaR1 and AvaR3. Each lane contains 6 ng of Paco probes and different amounts of proteins. (d) Identification of the repressive roles of Sav1778, Sav2268 and Sav2270 using Lux reporter system in E. coli. The values of relative light units (RLU) represent the average of at least three independent experiments. The output of the strain with plasmid pOacolux is set as control. For both (b) and (d), values are presented as mean ± SD from three independent experiments. Bridging lines show statistical details of comparisons between the control and the others. Asterisks indicate the statistically significant differences (*p < 0.05; **p < 0.01; ***p < 0.001).