Figure 1
From: CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury
Heat-induced temporal changes in CFTR and COX-2 expression in airway epithelial cells in vitro and in vivo.
(a,b) 16HBE14o- cells were cultured at 37 °C till 80% confluence before incubated at 52 °C for 5 min as heat-treatment and subsequently back at 37 °C for recovery. Cells were collected before (Ctrl), after heat-treatment (0 h), or after recovery for 8 hours (8 h) to 5 days (5d) for QRT-PCR (a) and western blot (b) analysis of CFTR and COX-2. Data are means ± SEM from at least three independent experiments. ***P < 0.001, **P < 0.01 as compared to Ctrl. n = 3. One-way ANOVA. (c,d) Representative images and corresponding semi-quantification of immunohistochemistry staining for CFTR (c) and COX-2 (d) in rat lung tissues 8 h-5d after inhalation of heated air. Same surgical procedures without the heat-inhalation were used in rats of the sham group. The intensity of CFTR and COX-2 positive signals was calibrated with the epithelial cell counts. ***P < 0.001, **P < 0.01 as compared to Ctrl. n = 3. One-way ANOVA. Bars = 100 μm (c,d).
