Figure 4
From: CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury
MAPKs activation precedes NF-κB signaling in response to heat or CFTR knockdown in 16HBE14o- cells.
(a,b) Western blotting for p-IκBα in cells with (+) or without (−) heat treatment (a), transfection of pEF/rib (b) in the presence (+) or absence (−) of PD98059 (20 μM), SP600125 (40 μM) or DMSO as vehicle control. Tubulin was used as loading controls. ###P < 0.001 as compared to cells without heat-treatment (a), or transfected with pEF (b). ***P < 0.001, **P < 0.01 as compared to DMSO-treated cells. n = 3, One-way ANOVA. (c–d) Western blotting for p-ERK, ERK, p-ERK and JNK in cells with (+) or without (−) heat-treatment (c) or transfection of pEFor rib (d) in the presence (+) or absence (−) of BAY11 (40 μM)or DMSO as vehicle control. Tubulin was used as loading controls. No visible effect of BAY11 was detected.
