Figure 5
From: CFTR-regulated MAPK/NF-κB signaling in pulmonary inflammation in thermal inhalation injury
IL-8 production as a result of MAPK/NF-κB/COX-2/PGE2 activation by heat or CFTR knockdown in 16HBE14o- cells.
(a,b) QRT-PCR analysis and ELISA detection of IL-8 production in cells with (+) or without (−) heat-treatment (a), transfection of pEF/rib (b) in the presence (+) or absence (−) of BAY11 (40 μM), PD98059 (20 μM), SP600125 (40 μM) or DMSO as vehicle control. ###P<0.001, ##P<0.01 as compared to cells without heat-treatment (a) or transfected with pEF (b). ***P<0.001, **P<0.01 as compared to DMSO-treated cells. n = 3, One-way ANOVA. (c) QRT-PCR analysis and ELISA detection of IL-8 in cells before (−) and after (+) heat-treatment in the presence (+) or absence (−) of the specific COX-2 inhibitor,NS-398 (10 μM) or DMSO as vehicle control. (d) ELISA detection of IL-8 in cells with or without treatment of VX-809.
