Figure 3
From: CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment
CD11b deficiency inactivated the Wnt/β-catenin pathway.
The expression of E-cadherin, β-catenin and cyclin D1 was examined in the tumor tissues of the ApcMin/+ and ApcMin/+;CD11b−/− mice by IHC (a) and immunoblotting (b) and the IHC results were quantitatively determined using IPP the software and expressed as the means ± S.D. The subcellular location of β-catenin was examined in the tumor tissues of the ApcMin/+ and ApcMin/+;CD11b−/− mice by IF (c). All IHC and IF staining in the mouse tissues represent 11 independent mice (all mice were 16-week-old). The IHC results were quantified using the IPP software and are expressed as the means ± S.D. **P < 0.01, ***P < 0.001. Scale bars, 20 μm (a–c) and 25 μm (e).
