Figure 3

Te2 (bregma −5.20 mm) neocortex was involved in remote memory retrieval when PSNs presented startled awakening.
(a,d,g) Photomicrographs of c-Fos staining in Te (bregma −4.52, −5.20 and −6.72 mm) cortical layers II-VI after normal and startled awakening, respectively. Scale bars = 200 μm. (b,c) The FSN and PSN groups did not exhibit significant differences in Te3 (bregma −4.52 mm). (e) The FSN group did not exhibit a significant difference in Te2 (bregma −5.20 mm). (f) In PSN rats, Fos counts significantly increased in layers II-III of Te2 (bregma −5.20 mm) compared with the control and PSC groups. (h,i) The FSN and PSN groups did not exhibit significant differences in Te2 (bregma −6.72 mm). The data are expressed as mean ± SEM. *p < 0.05, different from control; #p < 0.05, different from PSC. n = 6–7 per group. Te, temporal association cortex.