Figure 3
From: Heterogeneity of miR-10b expression in circulating tumor cells
MiR-10b ISH analysis of breast cancer cell lines captured by the CellSearch® system.
MiR-10b+ CK+ DAPI+ MCF-7 and MDA-MB-231 cells (white arrows) spiked into blood, captured by the CellSearch® system and identified by image analysis. Also shown are miR-10b− CK− DAPI+ (red arrows) and miR-10b+ CK− DAPI+ (yellow arrows) cells detected by IF microscopy. Also shown are the results U6-positive and scrambled-negative control probe analysis, respectively. Nuclei were visualized with DAPI. Resolution 40×. Scale bars, 20 μM.
