Figure 5 | Scientific Reports

Figure 5

From: Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection

Figure 5

Gene insertion in nucleofected cells.

The percentage of GFP-positive cells was quantified using flow cytometry during a 21-day culture after nucleofection with β-Ubc-GFP donor with and without (a) R02 CRISPR/Cas9 or (b) L4-R4 TALENs. The plots show normalized percentage of GFP-positive cells at specified days post nucleofection. Asterisks indicate significant difference between donor only and donor plus nuclease at specified days.

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