Figure 3 | Scientific Reports

Figure 3

From: Intron V, not intron I of human thrombopoietin, improves expression in the milk of transgenic mice regulated by goat beta-casein promoter

Figure 3

Identification of hTPO expression in the milk of transgenic mice by western blot.

Milk samples were diluted 1:2 in PBS and 20 μl of each sample was loaded under reducing conditions onto a SDS-PAGE gel (10%). Subsequent western blot was performed according to standard protocols. An HRP-conjugated anti-hTPO antibody from the kit diluted 1:300 was used. (a) Western blot. Lane 1: milk samples from non-transgenic mice; lane 2–8: milk samples from seven transgenic mice lines. (b) Band relative intensity of hTPO expression was quantified by Gel-Pro Analyzer 4.0 software.

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