Figure 5 | Scientific Reports

Figure 5

From: Intron V, not intron I of human thrombopoietin, improves expression in the milk of transgenic mice regulated by goat beta-casein promoter

Figure 5

Splicing as a requirement for intron V-mediated enhancement of hTPO transcription.

RT-PCR was performed to compare hTPO mRNA expression in HC-11 cells transfected with expression vector pTPOΔGD and pTPOGD, respectively. Intron V with a mutated 5′ splice site was not removed from hTPO pre-mRNA as indicated by the position of RT-PCR product.

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