Figure 3

The coating capacity of different treatments of B. cereus antigen.

(a) whole-cell lysates antigen prepared in 50 mM Tris-HCl, 10 mM EDTA, 100 mM NaCl, pH = 8.0, incubated for 30 min at 37 °C, followed by 150 W ultrasonication (10s, 10s, 90s cycle 40 times); (b) cell-surface extract antigen: antigen b1, B. cereus was in 5 mM Tris hydrochloride (TRISE), 1% (wt/vol) SDS and 5 mM 2-mercaptoethanol, pH = 9.8, heated to 70 °C for 30 min; antigen b2, B. cereus was in 5 mM Tris hydrochloride (TRISE) and 1% (wt/vol) SDS, pH = 8.0, incubated at room temperature for 30 min (c) antigen boiling: antigen was boiled in water for 10 min; (d) formaldehyde fixation of antigen: antigen was fixed in 4% formaldehyde for 20 min at room temperature; (e) paraformaldehyde fixation of antigen: antigen was fixed in 4% paraformaldehyde for 20 min at room temperature.